Journal: Stem Cells Translational Medicine

Article Title: Small Molecule Mesengenic Induction of Human Induced Pluripotent Stem Cells to Generate Mesenchymal Stem/Stromal Cells

doi: 10.5966/sctm.2011-0022

Figure Lengend Snippet: Morphology of ESC- and iPSC-derived mesenchymal stem/stromal cells (MSCs) by the embryoid body (EB) method. Shown is the morphology of the ESCs (A) and iPSCs (B) grown for 10 days in EB suspension culture (magnification, ×4). EBs were then transferred into tissue culture flasks with MSC medium, where they rapidly attached to the vessel ([C] and [D]; images are 4 days after attachment), after which the center of undifferentiated cells was removed by aspiration, and outgrowth cells were allowed to become confluent before further passaging. (E, F): ESC- and iPSC-derived MSCs form the EB method (mesenchymal passage 2) showed a standard MSC-like fibroblastic morphology. Abbreviations: ESC, embryonic stem cell; iPSC, induced pluripotent stem cell.

Article Snippet: Pluripotent Cell Culture Human iPSCs (MR90CL2 and ES4CL1 [ 27 ]) and ESCs (Mel1 and HES3 [ 28 ]) were supplied by StemCore (Australian Stem Cell Centre, Brisbane, Australia; www.aibn.uq.edu.au/pluripotent-stem-cell-core-facility ) under the appropriate human ethics and material transfer agreements. iPSCs and ESCs were routinely grown as bulk cultures on irradiated mouse embryonic fibroblasts (MEFs) (12,000 cells per cm 2 ) in medium consisting of DMEM-Ham's F-12 basal medium supplemented with 20% knockout serum replacement (KOSR), 1 mM l -glutamine, and 10 mM nonessential amino acids (all from Invitrogen).

Techniques: Derivative Assay, Suspension, Passaging

Journal: Stem Cells Translational Medicine

Article Title: Small Molecule Mesengenic Induction of Human Induced Pluripotent Stem Cells to Generate Mesenchymal Stem/Stromal Cells

doi: 10.5966/sctm.2011-0022

Figure Lengend Snippet: Gene expression profile of SB-induced differentiation of ESCs and iPSCs. (A): The expression of 53 genes selected from the quantitative reverse transcription-polymerase chain reaction array in ESC-derived and iPSC-derived MSCs. ESCs/iPSCs were cultured in mTeSR, KOSR medium in the presence of 10 μM SB for 10 days (KOSR+SB), and then after KOSR+SB, cells were subcultured in MSC medium and RNA extracted after one or two passages. Data are categorized into markers of pluripotency, germ layers (ectoderm, mesoderm, endoderm), hematopoietic cells, MSCs, and EMT. Expression levels were normalized to GAPDH and compared with those in mTeSR culture conditions. The heat map profile is presented as follows: red, high expression; white, medium expression; blue, low expression relative to overall expression over the three samples (time points) for a given probe. (B–E): Scatter plot comparisons of the gene expression profiles of the undifferentiated iPSC mTeSR condition with iPSC KOSR+SB (B) and iPSC-derived MSCs (iPS-MSCs) (D) at mesenchymal passage 1, and similarly of gene expression profiles of undifferentiated ESCs in mTeSR with ESC KOSR+SB (C) and ESC-derived MSCs (ES-MSCs) (E) at mesenchymal passage 2. Pink lines indicate boundaries of fourfold difference in gene expression, and highly expressed marker genes are indicated. Gene expression levels are depicted on a log10 scale. The scatter plots show the fold changes calculated using the 2−ΔCt formula. (F): iPSCs and ESCs were cultured on a Matrigel-coated plate in mTeSR medium (lanes 1 and 4) and treated with 10 μM SB in either mTeSR medium (lanes 2 and 5) or KOSR medium (lanes 3 and 6). After 10 days of treatment, the proteins in the cell lysates were subjected to Western blot analysis for SMAD2 (upper panel) and pSMAD2 (middle panel), and equal loading was assessed by Western blotting of the cell lysates for actin (bottom panel). Abbreviations: EMT, epithelial-to-mesenchymal transition; ESC, embryonic stem cell; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; iPSC, induced pluripotent stem cell; KOSR, knockout serum replacement; MSC, mesenchymal stem/stromal cell; SB, SB431542.

Article Snippet: Pluripotent Cell Culture Human iPSCs (MR90CL2 and ES4CL1 [ 27 ]) and ESCs (Mel1 and HES3 [ 28 ]) were supplied by StemCore (Australian Stem Cell Centre, Brisbane, Australia; www.aibn.uq.edu.au/pluripotent-stem-cell-core-facility ) under the appropriate human ethics and material transfer agreements. iPSCs and ESCs were routinely grown as bulk cultures on irradiated mouse embryonic fibroblasts (MEFs) (12,000 cells per cm 2 ) in medium consisting of DMEM-Ham's F-12 basal medium supplemented with 20% knockout serum replacement (KOSR), 1 mM l -glutamine, and 10 mM nonessential amino acids (all from Invitrogen).

Techniques: Gene Expression, Expressing, Reverse Transcription, Polymerase Chain Reaction, Derivative Assay, Cell Culture, Marker, Western Blot, Knock-Out

Journal: International review of neurobiology

Article Title: Cell transplantation to repair the injured spinal cord

doi: 10.1016/bs.irn.2022.09.008

Figure Lengend Snippet: Transplantation of tissues and cells to provide novel neuronal connections & an anatomical relay for spinal cord repair.

Article Snippet: S.Biomedics Co., Ltd. (Korea) , Neural precursor cells derived from human embryonic stem cell line , 18–65 y.o. SCI at C4-C7 level; AIS A , 7–60 days post-injury , Phase I and II , Safety; sensory and motor function evaluated with AIS, ISNCSCI, and SCIM scores , 2021–2028 , 5 , NCT04812431.

Techniques: Transplantation Assay, Suspension, Cell Culture

Journal: International review of neurobiology

Article Title: Cell transplantation to repair the injured spinal cord

doi: 10.1016/bs.irn.2022.09.008

Figure Lengend Snippet: Transplantation of tissues and cells to provide neuroprotection of spinal cord tissue.

Article Snippet: S.Biomedics Co., Ltd. (Korea) , Neural precursor cells derived from human embryonic stem cell line , 18–65 y.o. SCI at C4-C7 level; AIS A , 7–60 days post-injury , Phase I and II , Safety; sensory and motor function evaluated with AIS, ISNCSCI, and SCIM scores , 2021–2028 , 5 , NCT04812431.

Techniques: Transplantation Assay, Derivative Assay, Cell Culture

Journal: International review of neurobiology

Article Title: Cell transplantation to repair the injured spinal cord

doi: 10.1016/bs.irn.2022.09.008

Figure Lengend Snippet: Clinical trials transplanting tissue and cells for spinal cord repair.

Article Snippet: S.Biomedics Co., Ltd. (Korea) , Neural precursor cells derived from human embryonic stem cell line , 18–65 y.o. SCI at C4-C7 level; AIS A , 7–60 days post-injury , Phase I and II , Safety; sensory and motor function evaluated with AIS, ISNCSCI, and SCIM scores , 2021–2028 , 5 , NCT04812431.

Techniques: Clinical Proteomics, Modification, Derivative Assay, Transplantation Assay, Functional Assay, Control, Cell Culture

Journal: International review of neurobiology

Article Title: Cell transplantation to repair the injured spinal cord

doi: 10.1016/bs.irn.2022.09.008

Figure Lengend Snippet: Schematic diagram highlighting potential sources of neural stem and precursor cells: the developing neural tissues (A), pluripotent embryonic stem cells (B), and induced pluripotent stem cells (C; exemplified by skin fibroblast de-differentiation/reprogramming). These cells can be engineered to produce neural stem and progenitor cells that can then be expanded (D), specific subsets selected (e.g., NRPs and GRPs), and cryopreserved for “cell banking.” These cell stores can be thawed and prepared for transplantation into the injured spinal cord (E) alone, or in combination with additional treatments (E), including rehabilitation and activity-based therapy, neural interfacing and neuromodulation, application of scaffolds/biomaterials, or additional pharmaceuticals to enhance efficacy. Figure modified from Zholudeva, L. V., & Lane, M. A. (2022). Spinal interneurons: Plasticity after spinal cord injury, 1st ed. Academic Press.

Article Snippet: S.Biomedics Co., Ltd. (Korea) , Neural precursor cells derived from human embryonic stem cell line , 18–65 y.o. SCI at C4-C7 level; AIS A , 7–60 days post-injury , Phase I and II , Safety; sensory and motor function evaluated with AIS, ISNCSCI, and SCIM scores , 2021–2028 , 5 , NCT04812431.

Techniques: Transplantation Assay, Activity Assay, Modification

Journal: Cell Reports Methods

Article Title: Generation of dual-attribute iTNK cells from hPSCs for cancer immunotherapy

doi: 10.1016/j.crmeth.2024.100843

Figure Lengend Snippet: Expression of T cell signature genes in hPSC-derived T lineage cells (A) Uniform manifold approximation and projection (UMAP) representation of the T56 ATO T cell induction system containing T lineage, ILC lineage, mast cell lineage, and a small number of undefined clusters that did not resemble any in vivo cell type. Results from the hESC line H1 are shown. (B) Lineage-specific marker gene expression patterns in each cluster. (C) TCR + and TCR − cell distributions in the T56 ATO T cell induction system. (D) T cell signature and NK cell signature gene expression patterns in TCR + cells from the T56 ATO T cell induction system. See also Figures S2 and .

Article Snippet: human embryonic stem cell line H1 , iCell Bioscience Inc. , Cat#iCell-h299.

Techniques: Expressing, Derivative Assay, In Vivo, Marker, Gene Expression

Journal: Cell Reports Methods

Article Title: Generation of dual-attribute iTNK cells from hPSCs for cancer immunotherapy

doi: 10.1016/j.crmeth.2024.100843

Figure Lengend Snippet: Expression of typical T cell signature genes and NK cell signature genes in hPSC-derived iTNK cells (A) TCR + and TCR − cell distributions in hPSC-derived iTNK cells. Results from the hESC line H1 are shown. (B) Lineage-specific marker gene expression patterns in TCR + and TCR − cells. (C) T cell signature and NK cell signature gene expression patterns in TCR + iTNK cells. See also Figures S4 and .

Article Snippet: human embryonic stem cell line H1 , iCell Bioscience Inc. , Cat#iCell-h299.

Techniques: Expressing, Derivative Assay, Marker, Gene Expression

Journal: Cell Reports Methods

Article Title: Generation of dual-attribute iTNK cells from hPSCs for cancer immunotherapy

doi: 10.1016/j.crmeth.2024.100843

Figure Lengend Snippet: TCR repertoires and differences in expression of hPSC-derived T lineage cells and iTNK cells (A) CDR3 length distributions of hPSC-derived T lineage cells. Results from the hESC line H1 are shown. (B) CDR3 length distributions of hPSC-derived iTNK cells. Results from the hESC line H1 are shown. (C) TCR V-gene usage among hPSC-derived T lineage cells. (D) TCR V-gene usage among hPSC-derived iTNK cells. (E) Gene Ontology enrichment of upregulated genes in TCR + iTNK cells compared with TCR + T lineage cells. (F) Trajectory analysis of the cells in ATO system and iTNK cells. See also Figures S6–S8 .

Article Snippet: human embryonic stem cell line H1 , iCell Bioscience Inc. , Cat#iCell-h299.

Techniques: Expressing, Derivative Assay

Journal: Cell Reports Methods

Article Title: Generation of dual-attribute iTNK cells from hPSCs for cancer immunotherapy

doi: 10.1016/j.crmeth.2024.100843

Figure Lengend Snippet:

Article Snippet: human embryonic stem cell line H1 , iCell Bioscience Inc. , Cat#iCell-h299.

Techniques: Staining, Purification, Recombinant, Modification, Membrane, Cell Stimulation, Wright Stain, Software